Difference between revisions of "Part:BBa K567013"
Line 3: | Line 3: | ||
tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of lpp promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated. | tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of lpp promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated. | ||
+ | |||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K567013 parameters</partinfo> | ||
+ | <!-- --> | ||
===Construction of BBa_K567013=== | ===Construction of BBa_K567013=== | ||
Line 26: | Line 30: | ||
− | <!-- Uncomment this to enable Functional Parameter display | + | <!-- Uncomment this to enable Functional Parameter display |
− | + | ||
− | + | ||
− | + |
Revision as of 12:05, 5 October 2011
tRNA(Asp)-TAG
tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of lpp promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.
Functional Parameters
chassis | Escherichia coli |
Construction of BBa_K567013
This biobrick is constructed first by cloning the tRNAAsp from AspV in E.coli, then the anticodon region is site-directed mutated. This part is constructed on the backbone plasmid pACYC184.
Characterization of BBa_K567013
This part acts as a stop codon suppressor tRNA. It can be charged with Asp.
We have used Pbla-Luc-TAG as our Reporter. The amount of luciferase produced is reflected using the bioluminescence emitted during the luciferin reaction. Our results demonstrate that this part can suppress TAG insertion into luciferase. In the experimental group, with the help of BBa_K567011 PT7-TDRS (Favorite Part), luciferase-TAG was produced and bioluminescence was emitted during the luciferin reaction. These results proved that this tRNAAsp can effectively suppress stop codon.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 281
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 281
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 281
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 281
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 281
- 1000COMPATIBLE WITH RFC[1000]