Difference between revisions of "Part:BBa K658004"

Revision as of 11:31, 5 October 2011

a bacteria population-control device with RBS0.3 driven by lacl+pL

The device is constructed based on the Biobrick part BBa_K658003. This device is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 718
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2345
Illegal BsaI.rc site found at 1874

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 __NOTOC__
 <partinfo>BBa_K658004 short</partinfo>
-The device is a twin part of Biobrick part BBa_K658001. This device is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.
+The device is constructed based on the Biobrick part BBa_K658003. This device is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.