Difference between revisions of "Part:BBa K524007"

 
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__NOTOC__
 
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<partinfo>BBa_K524007 short</partinfo>
 
<partinfo>BBa_K524007 short</partinfo>
  
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The split sfGFP11 was constructed from an pBAD driven sfGFP BBa_I746908. This part is just the CDS of the spilt sfGFP 11.
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The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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When driven by an appropriate promoter, the product of this biobrick should complement with the product of biobrick sfGFP11 [https://parts.igem.org/Part:BBa_K524006 BBa_K524006] to give green fluorescence under UV.
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Revision as of 10:45, 5 October 2011

RBS+ split sfGFP 11 + double terminator

The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.

Usage and Biology

When driven by an appropriate promoter, the product of this biobrick should complement with the product of biobrick sfGFP11 BBa_K524006 to give green fluorescence under UV.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]