Difference between revisions of "Part:BBa K524001"

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(→‎Usage and Biology)
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The split sfGFP1-10 in this biobrick is assembled behind R0010 Lac promoter.
 
The split sfGFP1-10 in this biobrick is assembled behind R0010 Lac promoter.
  
−
This product of this biobrick should complement with the product of biobrick sfGFP11 [https://parts.igem.org/Part:BBa_K524002 BBa_K524002] to give green fluorescence under UV.
+
Induced by IPTG, the product of this biobrick should complement with the product of IPTG inducible biobrick sfGFP11 [https://parts.igem.org/Part:BBa_K524002 BBa_K524002] to give green fluorescence under UV.
  
 
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Revision as of 10:44, 5 October 2011

pLac + RBS + split sfGFP 1-10

The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.

Usage and Biology

The split sfGFP1-10 in this biobrick is assembled behind R0010 Lac promoter.

Induced by IPTG, the product of this biobrick should complement with the product of IPTG inducible biobrick sfGFP11 BBa_K524002 to give green fluorescence under UV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238