Difference between revisions of "Part:BBa K524006"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K524006 short</partinfo>
 
<partinfo>BBa_K524006 short</partinfo>
  
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The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. This part is the CDS of it.
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The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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When driven by an appropriate promoter, the product of this biobrick should complement with the product of biobrick sfGFP11 [https://parts.igem.org/Part:BBa_K524007 BBa_K524007] to give green fluorescence under UV.
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Revision as of 10:40, 5 October 2011

RBS + split sfGFP1-10

The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.

Usage and Biology

When driven by an appropriate promoter, the product of this biobrick should complement with the product of biobrick sfGFP11 BBa_K524007 to give green fluorescence under UV.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 31