Difference between revisions of "Part:BBa K598003:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K598003 short</partinfo>
 
<partinfo>BBa_K598003 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Only use it by sites mutation
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This part is a thiamine pyrophosphate (TPP) ligand responsive ribozyme numbered 2.5 with native RBS (AAGGAGAT), which can inhibit gene expression responding to TPP. Although there are standard restriction sites in this part, attaching upstream of target gene can only be operated by site-directed mutation. Because the spacing between RBS and start code of target gene after standard assembly is not appropriate.
  
  
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===Source===
 
===Source===
  
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E.coli
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This part is obtained by PCR from inactive TPP-HHAZ 2.5 which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.  
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+
 
===References===
 
===References===
 +
Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756

Latest revision as of 08:54, 5 October 2011

TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a thiamine pyrophosphate (TPP) ligand responsive ribozyme numbered 2.5 with native RBS (AAGGAGAT), which can inhibit gene expression responding to TPP. Although there are standard restriction sites in this part, attaching upstream of target gene can only be operated by site-directed mutation. Because the spacing between RBS and start code of target gene after standard assembly is not appropriate.


Source

This part is obtained by PCR from inactive TPP-HHAZ 2.5 which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.

References

Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756