Difference between revisions of "Part:BBa K649301"

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This work is done by Natsuki Kubo.]]
 
This work is done by Natsuki Kubo.]]
  
Introduction of BBa_K649301 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +). This part produced urea more efficiently in strain JD24293 (<span style="font-style:italic;">argR</span> -).
+
Introduction of BBa_K649301 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +). This part produced urea more efficiently in strain JD24293 (<span style="font-style:italic;">argR</span> -).<br /><br />
 +
For more information, see[http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm. our work in Tokyo_Tech 2011 wiki.]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:45, 5 October 2011

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

Urea concentration in growth media 1 hour after IPTG induction.
This work is done by Natsuki Kubo.

Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +). This part produced urea more efficiently in strain JD24293 (argR -).

For more information, see[http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm. our work in Tokyo_Tech 2011 wiki.]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]