Difference between revisions of "Part:BBa K539691:Experience"
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Revision as of 08:13, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K539691
Experience by 2011 iGEM NCTU_FORMOSA
Figure.1 Firstly , we ligase B0030 with ilvC.
Figure.2 At the sametime , we ligase B0030 with ilvD.
Figure.3 Meanwhile , we ligase B0032 with Alss.
Figure.4 To make sure we obtain the correct part , colony PCR is needed.
Figure.5 Secondly , we ligase Plac with B0032+Alss
Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC
Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator
Figure.8 Finally , we ligase the whole circuit:promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator
To make sure our design could work , we cotransform Part:BBa_K539691 and Part:BBa_K539742 into DH5α, so the expression of alss, ilvC, ilvD and kivd are under the control of temperature.
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