Difference between revisions of "Part:BBa K567016:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ===Construction of BBa_K567016=== |
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| + | ''metY''-CGA: We have cloned operon ''metY'' containing tRNA<sup>Met</sup> from ''E.coli'' to pACYC184. The anticodon of tRNA<sup>Met</sup> was mutated to TCG (base pairing codon CGA). | ||
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| + | ===Characterization of BBa_K567016=== | ||
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| + | When this part, MetRS PT7-''metG''N (BBa_K567015)(or MetRS PT7-''metG''M (BBa_K567014)) and KanaR (BBa_K567020) are co-transformed into the cell, the cell is expected to survive Kana. | ||
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| + | [[image:11SJTU-initial_codon_result.jpg|frame|center|fig. Growth of ER2566 with a. ''metG''N + ''metY''-CGA, b. ''metG''M + ''metY''-CGA, c. + ''metG''N, d. + ''metG''M. Growth medium (left): LB Kana+Tet. Growth medium (right): LB Kana.]] | ||
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| + | Cell growth shows that the cells show Kana resistance only when both modified MetRS (''metG''N) and modified tRNA<sup>Met</sup>(''metY''-CGA) are transformed into the cell, proving that ''metG''N works well. | ||
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| + | For more information concerning this part, please see [http://2011.igem.org/Team:SJTU-BioX-Shanghai 2011 SJTU-BioX-iGEM] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 08:11, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Construction of BBa_K567016
metY-CGA: We have cloned operon metY containing tRNAMet from E.coli to pACYC184. The anticodon of tRNAMet was mutated to TCG (base pairing codon CGA).
Characterization of BBa_K567016
When this part, MetRS PT7-metGN (BBa_K567015)(or MetRS PT7-metGM (BBa_K567014)) and KanaR (BBa_K567020) are co-transformed into the cell, the cell is expected to survive Kana.
Cell growth shows that the cells show Kana resistance only when both modified MetRS (metGN) and modified tRNAMet(metY-CGA) are transformed into the cell, proving that metGN works well.
For more information concerning this part, please see [http://2011.igem.org/Team:SJTU-BioX-Shanghai 2011 SJTU-BioX-iGEM]
User Reviews
UNIQ892bdfa59b02fd68-partinfo-00000000-QINU UNIQ892bdfa59b02fd68-partinfo-00000001-QINU