Difference between revisions of "Part:BBa K581001:Design"

(→‎Design Notes)
(→‎References)
 
Line 18: Line 18:
  
 
===References===
 
===References===
 +
 +
Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61, 1013-1022.

Latest revision as of 02:42, 5 October 2011

ptsG1-GFP (ptsG1 5' UTR fused with gfp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747


Design Notes

This part is designed as the C85G mutant of the ptsG(wt)'s 5' untranslated region fused with gfp.

Teppei Morita et.al’ s work suggests that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. Since a specific repression effect is favored in our comparator device, the characteristics of the conjugate mutated ptsG/SgrS system meet the need quite well.

Also, such a site mutation is designed for biological orthogonality.

Source

Escherichia coli genome.

References

Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61, 1013-1022.