Difference between revisions of "Part:BBa K581000:Design"

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===Design Notes===
 
===Design Notes===
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Site specifically mutated for biological orthogonality. Besides, the design of G178C site mutation is also aimed at restoring its repression effection against C87G mutant ptsG mRNA.
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This is the G178C mutant of the small RNA SgrS(wt).
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Teppei Morita '''et.al'''’ s work suggest that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. Therefore, such a site mutation is designed for biological orthogonality.
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Also, since a specific repression effect is favored in our comparator device, the characteristics of the conjugate mutated ptsG/SgrS system meet the need quite well.
  
 
===Source===
 
===Source===

Revision as of 02:33, 5 October 2011

sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the G178C mutant of the small RNA SgrS(wt).

Teppei Morita et.al’ s work suggest that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. Therefore, such a site mutation is designed for biological orthogonality.

Also, since a specific repression effect is favored in our comparator device, the characteristics of the conjugate mutated ptsG/SgrS system meet the need quite well.

Source

E.coli K-12 strain.

References