Difference between revisions of "Part:BBa K646000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [[Image:MQ_New_data_page_diagram.jpg|center|750px]] <br> '''The BioBrick part in team Macquarie University (2011) mechanism'''<br><br> | ||
When coupled to a promoter BioBrick such as T7 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]) to produce parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646003 BBa_K646003] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646004 BBa_K646004], and transformed into Bl21(DE3)Tuner ''E. coli'' cells, a green colouration (require induction with ALA and IPTG) is produced. | When coupled to a promoter BioBrick such as T7 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]) to produce parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646003 BBa_K646003] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646004 BBa_K646004], and transformed into Bl21(DE3)Tuner ''E. coli'' cells, a green colouration (require induction with ALA and IPTG) is produced. | ||
Revision as of 01:41, 5 October 2011
Hemeoxygenase with RBS
Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.
Usage and Biology
The BioBrick part in team Macquarie University (2011) mechanism
When coupled to a promoter BioBrick such as T7 promoter (BBa_I719005 and BBa_I712074) to produce parts BBa_K646003 and BBa_K646004, and transformed into Bl21(DE3)Tuner E. coli cells, a green colouration (require induction with ALA and IPTG) is produced.
Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green as heme oxyganse was expressed using T7 promoter and degraded heme into biliverdin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]