Difference between revisions of "Part:BBa K646003"

Revision as of 08:35, 4 October 2011 (view source) Eddymoh (Talk | contribs) (→‎Usage and Biology) ← Older edit		Revision as of 01:39, 5 October 2011 (view source) Red Dave (Talk | contribs) Newer edit →
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	===Usage and Biology===		===Usage and Biology===
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		+	[[Image:MQ_New_data_page_diagram.jpg|center|500px]] <br> The BioBrick part in team Macquarie University (2011) mechanism
	[[Image:Results_-_green colonies.JPG|400px]]		[[Image:Results_-_green colonies.JPG|400px]]

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Revision as of 01:39, 5 October 2011

T7 promoter with Hemeoxygenase

Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI.

Successful expression should result in green colonies due to the presence of biliverdin.

Usage and Biology

The BioBrick part in team Macquarie University (2011) mechanism

Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green as heme oxyganse was expressed using T7 promoter and degraded heme into biliverdin.

Sequence and Features