Difference between revisions of "Part:BBa K649105"

Revision as of 00:48, 5 October 2011

PlsrA-gfp-PlsrR-lsrR

Fluorescence intensity is decreased by LsrR repression.
This work is done by Hiroki Yoshise.

As we described in BBa_K649101, in this part, lsrK has mutation and does not work properly and oher genes work correctly.

We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a lsrR gene downstream of lsrA promoter-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed promoter lsrA. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. our work in Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2579
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2253
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 770

@@ Line 5: / Line 5: @@
 [[Image:Lsrr.png|thumb|center|500px|Fluorescence intensity is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]
-This part is described as PlsrA-gfp-PlsrR-lsrR because lsrK of BBa_649101 has mutation and does not work properly.
+As we described in [https://parts.igem.org/Part:BBa_K649101 BBa_K649101], in this part, lsrK has mutation and does not work properly and oher genes work correctly.
 We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a lsrR gene downstream of lsrA promoter-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed promoter lsrA. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.