Difference between revisions of "Part:BBa K649101"
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PlsrR and lsrR work correctly. | PlsrR and lsrR work correctly. | ||
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However, in this part,lsrK has mutation and does not work properly. | However, in this part,lsrK has mutation and does not work properly. | ||
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LsrR is the repressor of the lsr operon and itself. | LsrR is the repressor of the lsr operon and itself. | ||
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To confirm LsrR represses lsrA promoter, we constructed BBa_K649105. If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649105 here.] | To confirm LsrR represses lsrA promoter, we constructed BBa_K649105. If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649105 here.] | ||
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For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki] | For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki] | ||
Revision as of 00:43, 5 October 2011
PlsrR-lsrR
PlsrR and lsrR work correctly.
However, in this part,lsrK has mutation and does not work properly.
LsrR is the repressor of the lsr operon and itself.
To confirm LsrR represses lsrA promoter, we constructed BBa_K649105. If you want to see detailed infomation, please click here.
For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1725
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1399
- 1000COMPATIBLE WITH RFC[1000]