Difference between revisions of "Part:BBa K574009:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K574009=== | ===Applications of BBa_K574009=== | ||
| + | '''Description:''' we assembled E0840 under the pLas promoter that was contained into K574009. We kept pSB1A2 as the scaffold vector. | ||
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| + | '''Methods:''' we ligated E0840 downstream of K574009, and the positive colony was cultured at 37°C, 220 rpm for 12 hours, as well as the DH5alpha. Then we diluted 1:25 the former in 15 falcon tubes (4 ml cultures), each 3 tubes as a group. The later was also diluted in 3 tubes as the negative control. After Cultured for another 2 hours, the groups were induced with 3OC12HSL 0, 10^-6M, 10^-5M and 10^-4M respectively. We took samples after 1 hours, 4 hours, 8 hours and 24 hours, and then the samples were measured through the flow cytometer. | ||
| + | <br> | ||
| + | '''Results:''' From the chart, we can see that cells were hardly induced in the control group, and with the concentration of inducer growing, the intensity of GFP increased by groups. The most efficient concentration of inducer was around 10^-6M, and to most groups, the intensity of GFP reached its maxium after 4 hours. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:38, 4 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K574009
Description: we assembled E0840 under the pLas promoter that was contained into K574009. We kept pSB1A2 as the scaffold vector.
Methods: we ligated E0840 downstream of K574009, and the positive colony was cultured at 37°C, 220 rpm for 12 hours, as well as the DH5alpha. Then we diluted 1:25 the former in 15 falcon tubes (4 ml cultures), each 3 tubes as a group. The later was also diluted in 3 tubes as the negative control. After Cultured for another 2 hours, the groups were induced with 3OC12HSL 0, 10^-6M, 10^-5M and 10^-4M respectively. We took samples after 1 hours, 4 hours, 8 hours and 24 hours, and then the samples were measured through the flow cytometer.
Results: From the chart, we can see that cells were hardly induced in the control group, and with the concentration of inducer growing, the intensity of GFP increased by groups. The most efficient concentration of inducer was around 10^-6M, and to most groups, the intensity of GFP reached its maxium after 4 hours.
User Reviews
UNIQ3dcd6948e6c43c6b-partinfo-00000000-QINU UNIQ3dcd6948e6c43c6b-partinfo-00000001-QINU