Difference between revisions of "Part:BBa K649201:Design"

(→‎References)
 
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
The forward lox2272 is annealed oligonucleotide and the following lox2272 was produced by PCR (template BBa_J04450).
+
The forward lox2272 is synthetic oligo and the following lox2272 was produced by PCR (template BBa_J04450).
 
The information of lox2272 sequence was gain from the reference below.
 
The information of lox2272 sequence was gain from the reference below.
  
 
===References===
 
===References===
−
Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.
+
Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki ''et al'', Nucleic Acids Research 2002.

Latest revision as of 15:28, 4 October 2011

PlacIQ-lox2272-rfp-lox2272-gfp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1016
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 686
    Illegal AgeI site found at 798
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1700


Design Notes

PlacIQ + lox2272 + RBS + mRFP + T7 terminator + lox2272 +RBS + GFP + T7

Source

The forward lox2272 is synthetic oligo and the following lox2272 was produced by PCR (template BBa_J04450). The information of lox2272 sequence was gain from the reference below.

References

Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.