Difference between revisions of "Part:BBa K539691:Experience"
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'''Figure.1 Firstly , we ligase B0030 with ilvC.''' | '''Figure.1 Firstly , we ligase B0030 with ilvC.''' | ||
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[[Image:butanol_figure2.jpg]] | [[Image:butanol_figure2.jpg]] | ||
'''Figure.2 At the sametime , we ligase B0030 with ilvD.''' | '''Figure.2 At the sametime , we ligase B0030 with ilvD.''' | ||
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[[Image:butanol_figure3.jpg]] | [[Image:butanol_figure3.jpg]] | ||
'''Figure.3 Meanwhile , we ligase B0032 with Alss.''' | '''Figure.3 Meanwhile , we ligase B0032 with Alss.''' | ||
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[[Image:butanol_figure4.jpg]] | [[Image:butanol_figure4.jpg]] | ||
'''Figure.4 To make sure we obtain the correct part , colony PCR is needed.''' | '''Figure.4 To make sure we obtain the correct part , colony PCR is needed.''' | ||
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[[Image:butanol_figure5.jpg]] | [[Image:butanol_figure5.jpg]] | ||
'''Figure.5 Secondly , we ligase Plac with B0032+Alss''' | '''Figure.5 Secondly , we ligase Plac with B0032+Alss''' | ||
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[[Image:butanol_figure6.jpg]] | [[Image:butanol_figure6.jpg]] | ||
'''Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC''' | '''Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC''' | ||
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[[Image:butanol_figure7.jpg]] | [[Image:butanol_figure7.jpg]] | ||
'''Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator''' | '''Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator''' | ||
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[[Image:butanol_figure8.jpg]] | [[Image:butanol_figure8.jpg]] | ||
Revision as of 15:11, 4 October 2011
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Applications of BBa_K539691
Figure.1 Firstly , we ligase B0030 with ilvC.
Figure.2 At the sametime , we ligase B0030 with ilvD.
Figure.3 Meanwhile , we ligase B0032 with Alss.
Figure.4 To make sure we obtain the correct part , colony PCR is needed.
Figure.5 Secondly , we ligase Plac with B0032+Alss
Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC
Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator
Figure.8 Finally , we ligase the whole circuit:promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator
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