Difference between revisions of "Part:BBa K574006:Design"

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===Design Notes===
 
===Design Notes===
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We design this part to regulate the sending of the 3OC12HSL. L-arabinose acts as an inducer which we can add into the system and TetR acts as a repressor which comes from the system inside.
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===Source===
 
===Source===

Latest revision as of 14:31, 4 October 2011

3OC12HSL regulated by pTet and pBad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 744
    Illegal BglII site found at 1625
    Illegal BamHI site found at 870
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
    Illegal AgeI site found at 1187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this part to regulate the sending of the 3OC12HSL. L-arabinose acts as an inducer which we can add into the system and TetR acts as a repressor which comes from the system inside.

Source

We used BioBrick Standard Assembly. List as follows: BBa_R0040 BBa_K081016 BBa_I13453 BBa_K081009 BBa_E0430

References