Difference between revisions of "Part:BBa K649200"
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lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise.
 
lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise.
 
The Cre-mediated recombination of this BioBrick had been studied by ''in vitro'' assay, and proved to be working.
 
The Cre-mediated recombination of this BioBrick had been studied by ''in vitro'' assay, and proved to be working.
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[[Image:in vitro_lox_gfp_lox.png|thumb|center|500px|<br/>|''in vitro'' assay of K649200<br />negative control, 0.5hr, 2hr, and 4hr from left]]
  
 
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Revision as of 09:16, 4 October 2011

PlacIQ-lox2272-gfp-lox2272

lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied by in vitro assay, and proved to be working.

in vitro assay of K649200
negative control, 0.5hr, 2hr, and 4hr from left

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790