Difference between revisions of "Part:BBa K646004"
| Line 6: | Line 6: | ||
Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1). | Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1). | ||
| − | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | [[Image:Results_-_green colonies.JPG|400px]] | ||
| + | |||
| + | Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green as heme oxyganse was expressed using T7 promoter and degraded heme into biliverdin. | ||
<!-- --> | <!-- --> | ||
Revision as of 08:36, 4 October 2011
T7 promoter with Hemeoxygenase
Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available.
Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1).
Usage and Biology
Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green as heme oxyganse was expressed using T7 promoter and degraded heme into biliverdin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]