Difference between revisions of "Part:BBa K649402"

Revision as of 08:36, 4 October 2011

Ptrc-RBS-rocF-Arg box

This part contains Ptrc & RBS & rocF & Arg box.

Urea concentration in growth media 1 hour after IPTG induction.
This work is done by Natsuki Kubo.

Introduction of BBa_K649402 containing arginase coding gene (rocF) and Arg Box (an arg operator capable of binding the arginine repressor) led to production of some urea in strain MG1655 (argR +) and strain JD24293 (argR -). But in both strains, the amount urea produced was not much different from that when BBa_K649301 containing rocF but not Arg box) was introduced. This result shows that rocF is working but Arg box is not working efficiently in this part. The reason why Arg box is not working is probably that low-copy-number plasmid used here is not capable of introducing enough number of Arg boxes to effectively deactivate the arginine repressor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 230
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part contains Ptrc & RBS & rocF & Arg box.
-[[Image:BBa K649402 graph(2).png|thumb|center|500px|Introduction of BBa_K649402 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) and Arg Box (an <span style="font-style:italic;">arg</span> operator capable of binding the arginine repressor) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +) and strain JD24293 (<span style="font-style:italic;">argR</span> -). But in both strains, the amount urea produced was not much different from that when BBa_K649301 containing <span style="font-style:italic;">rocF</span> but not Arg box) was introduced. This result shows that <span style="font-style:italic;">rocF</span> is working but Arg box is not working efficiently in this part.  The reason why Arg box is not working is probably that low-copy-number plasmid used here is not capable of introducing enough number of Arg boxes to effectively deactivate the arginine repressor.<br />
+[[Image:BBa K649402 graph(2).png|thumb|center|500px|Urea concentration in growth media 1 hour after IPTG induction.<br />
 This work is done by Natsuki Kubo.]]
+Introduction of BBa_K649402 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) and Arg Box (an <span style="font-style:italic;">arg</span> operator capable of binding the arginine repressor) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +) and strain JD24293 (<span style="font-style:italic;">argR</span> -). But in both strains, the amount urea produced was not much different from that when BBa_K649301 containing <span style="font-style:italic;">rocF</span> but not Arg box) was introduced. This result shows that <span style="font-style:italic;">rocF</span> is working but Arg box is not working efficiently in this part.  The reason why Arg box is not working is probably that low-copy-number plasmid used here is not capable of introducing enough number of Arg boxes to effectively deactivate the arginine repressor.