Difference between revisions of "Part:BBa K649201"

Revision as of 04:47, 4 October 2011

PlacIQ-lox2272-rfp-lox2272-gfp

lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.

Effect of Cre-meditated recombination at lox2272 cassette.
The leftmost is a negative control which don't have Cre-expressing plasmid.
The center is an arabinose induced sample which has both Cre plasmid and BioBrick BBa_K649201.
The rightmost is a uninduced strain which has both plasmid like as the center.

The uninduced sample having both plasmid was supposed to unexpress Cre recombinase, but because of the leaking of PBAD\araC promoter, the excision occured in almost every cell in medium.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1016
Illegal BamHI site found at 14
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 686
Illegal AgeI site found at 798
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1700

@@ Line 4: / Line 4: @@
 lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.
-[[Image:111001DK_0.5_2272.png|thumb|center|500px|Effect of Cre-meditated recombination at lox2272 cassette.<br />The leftmost is a negative control which don't have Cre-expressing plasmid. The Center is an arabinose induced sample which has both Cre plasmid and BioBrick BBa_K649201. The rightmost is a uninduced strain which has both plasmid like as the center.
+[[Image:111001DK_0.5_2272.png|thumb|center|500px|Effect of Cre-meditated recombination at lox2272 cassette.<br />The leftmost is a negative control which don't have Cre-expressing plasmid. <br />The center is an arabinose induced sample which has both Cre plasmid and BioBrick BBa_K649201. <br />The rightmost is a uninduced strain which has both plasmid like as the center.
 ]]