Difference between revisions of "Part:BBa K649201"

Revision as of 04:22, 4 October 2011

PlacIQ-lox2272-rfp-lox2272-gfp

lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1016
Illegal BamHI site found at 14
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 686
Illegal AgeI site found at 798
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1700

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 lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied and proved to be working.
-[[Image:111001DK_0.5_2272.png|thumb|center|500px|Effect of Cre recombinase induced by arabinose.<br />The leftmost is a negative control which don`t have Cre-expressing plasmid. The Center is a arabinose induced sample which has both Cre plasmid and BioBrick BBa_K649201. The rightmost is a uninduced strain which has both plasmid like as the center.| |]]
+[[Image:111001DK_0.5_2272.png|thumb|center|500px|Effect of Cre recombinase induced by arabinose.<br />The leftmost is a negative control which don`t have Cre-expressing plasmid. The Center is a arabinose induced sample which has both Cre plasmid and BioBrick BBa_K649201. The rightmost is a uninduced strain which has both plasmid like as the center. |]]
 <!-- Add more about the biology of this part here