Difference between revisions of "Part:BBa K524002"
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| − | This product of this biobrick should complement with the product of biobrick [ | + | This product of this biobrick should complement with the product of biobrick [https://parts.igem.org/Part:BBa_K524001 BBa_K524001] to give green fluorescence under UV. |
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Revision as of 19:38, 3 October 2011
pLac + RBS+ split sfGFP11 + double terminator
The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 215-238 of sfGFP. Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
Usage and Biology
This product of this biobrick should complement with the product of biobrick BBa_K524001 to give green fluorescence under UV.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]