Difference between revisions of "Part:BBa K524001:Design"

(→‎Design Notes)
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Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107
 
Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107
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Stéphanie Cabantous & Geoffrey S Waldo.(2006).In vivo and in vitro protein solubility assays using split GFP.Nature Methods - 3, 845 - 854

Revision as of 19:23, 3 October 2011

pLac + RBS + split sfGFP 1-10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238


Design Notes

A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10. RBS was introduced into primers for cloning.

Source

Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908

References

Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107

Stéphanie Cabantous & Geoffrey S Waldo.(2006).In vivo and in vitro protein solubility assays using split GFP.Nature Methods - 3, 845 - 854