Difference between revisions of "Part:BBa K518004:Experience"

Revision as of 18:51, 3 October 2011

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Applications of BBa_K518004

User Reviews

UNIQc1f0ae6031afc11f-partinfo-00000000-QINU

UT-Tokyo 2011

We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (1), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin assay and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo |our result page].

UNIQc1f0ae6031afc11f-partinfo-00000002-QINU

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 <I>UT-Tokyo 2011</I>
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-We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but could not. Our sequencing results indicated a general sequence agreement, showing only a few mismatching bases. We have tried to detect the L-Asp by either ninhydrin staining or ultraviolet-visible spectroscopy, only to fail. Ninhydrin reactedwith not only amino acid (Asp) but indeed ammonium ion itself, and U-V spectroscopy detected large fumarate noise. We also tried to directly measure over-expressed intracellular AspA, but had few days to do this work.
+We have worked hard to demonstrate that this part enables bacteria to produce L-aspartate, but failed to do that. In the previous works (1), the amount of L-Asp was determined by HPLC. We, however, were unable to use this machinery, so we tried to detect it through alternative method, ninhydrin assay and ultraviolet-visible spectroscopy. For experimental details, see [http://2011.igem.org/Team:UT-Tokyo |our result page].
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