Difference between revisions of "Part:BBa K649200:Experience"
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===Applications of BBa_K649200=== | ===Applications of BBa_K649200=== | ||
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The ''in vitro'' assay of BBa_640200 allowed us to confirm that the Cre-mediated recombination on lox2272 cassette works as designed. In the assay, the part was made linear using restriction enzymes. Cre recombinase (1,000 units/ml, 1μl) was added to the linear DNA (50ng/μl, 4 μl) and incubated for 0.5, 2, and 4 hours. Images of the experiments have been added below. | The ''in vitro'' assay of BBa_640200 allowed us to confirm that the Cre-mediated recombination on lox2272 cassette works as designed. In the assay, the part was made linear using restriction enzymes. Cre recombinase (1,000 units/ml, 1μl) was added to the linear DNA (50ng/μl, 4 μl) and incubated for 0.5, 2, and 4 hours. Images of the experiments have been added below. | ||
Revision as of 13:04, 3 October 2011
Applications of BBa_K649200
The in vitro assay of BBa_640200 allowed us to confirm that the Cre-mediated recombination on lox2272 cassette works as designed. In the assay, the part was made linear using restriction enzymes. Cre recombinase (1,000 units/ml, 1μl) was added to the linear DNA (50ng/μl, 4 μl) and incubated for 0.5, 2, and 4 hours. Images of the experiments have been added below.
IMAGES
These images of the electrophoresis experiments show that there are several bands in samples to which Cre was added, which indicates that excision of the lox sites successfully occurred.
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