Difference between revisions of "Part:BBa K117002:Experience"

Revision as of 10:53, 3 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K117002

This promoter is activated indirectly by AI-2 to promote whatever downstream gene ligated behind it.

Characterisation:

For information on characterisation of this new part, please visit Part K117010 Experience and Part K117008 Experience

User Reviews

UNIQee4331adb3b1231c-partinfo-00000000-QINU UNIQee4331adb3b1231c-partinfo-00000001-QINU

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Tokyo Tech 2011

This part(BBa_K117002) does not work properly. To confirm this, we introduced a gfp gene(BBa_J54103) downstream of the promoter. As a consequence, fluorescence intensity of promoter lsrA(BBa_K117002)-gfp was almost the same as promoterless-gfp(negative control), showing that promoter promoter lsrA(BBa_K117002) does not work properly.

We improved this part.Our new promoter lsrA(BBa_K649100) works.

If you want to know why our part works, click [here] and visit our team's wiki.

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 <I>Tokyo Tech 2011</I>
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+This part(BBa_K117002) does not work properly. To confirm this, we introduced a gfp gene(BBa_J54103) downstream of the promoter. As a consequence, fluorescence intensity of promoter lsrA(BBa_K117002)-gfp was almost the same as promoterless-gfp(negative control), showing that promoter promoter lsrA(BBa_K117002) does not work properly.
+We improved this part.Our new promoter lsrA(BBa_K649100) works.
+If you want to know why our part works, click [here] and visit our team's wiki.
 |};