Difference between revisions of "Part:BBa K598010:Design"
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===Source=== | ===Source=== | ||
| − | + | BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive promoter library. | |
| + | RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation. | ||
===References=== | ===References=== | ||
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614. | Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614. | ||
Revision as of 07:24, 3 October 2011
pBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal XhoI site found at 209 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 868
Design Notes
Check the RBS sequence before using.
Source
BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive promoter library. RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.
References
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.