Difference between revisions of "Part:BBa K598005:Design"
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− | + | Sequence is based on original design of Beatrix Suess. Insert RNA controller sequence into backbone via PCR and bluntend ligation. | |
===References=== | ===References=== | ||
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression ''in vivo''. Nucleic Acids Research ''32'', 1610-1614. | Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression ''in vivo''. Nucleic Acids Research ''32'', 1610-1614. |
Revision as of 05:37, 3 October 2011
Theophylline Responsive Riboswitch P1G1 with Native RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a test to add a new part.3
Source
Sequence is based on original design of Beatrix Suess. Insert RNA controller sequence into backbone via PCR and bluntend ligation.
References
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.