Difference between revisions of "Part:BBa K578001"
Ethan ward (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K578001 short</partinfo> <partinfo>BBa_K578001</partinfo>. ===Usage and Biology=== * This is the AT-rich leader region of the CRISPR I array in Escherichia coli s...) |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K578001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K578001 SequenceAndFeatures</partinfo> | ||
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+ | ===Confirmation of Part Length=== | ||
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+ | [[Image: ASU_K12_Leader.png|400px]] |
Revision as of 23:44, 2 October 2011
E. coli CRISPR I leader sequence
Usage and Biology
- This is the AT-rich leader region of the CRISPR I array in Escherichia coli str. K-12 substr. MG1655. "Identification and characterization of E. coli CRISPR-cas promoters and their silencing by H-NS" (Ü. Pul et al, 2010) characterized a single promoter (Pcrispr1) in this sequence. Transcription is silenced through H-NS binding to the leader sequence. Induction requires anti-silencing, either by protein-independent processes, DNA-binding proteins or other modulatory mechanisms not yet identified.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]