Difference between revisions of "Part:BBa K578002"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K578002 short</partinfo> | <partinfo>BBa_K578002 short</partinfo> | ||
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<partinfo>BBa_K578002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K578002 SequenceAndFeatures</partinfo> | ||
+ | <br> | ||
+ | ===Confirmation of Part Length=== | ||
+ | |||
+ | [[Image:ASU_T7LRSR.png|400px]] | ||
+ | |||
+ | ===Use of this BioBrick=== | ||
+ | This part has been [http://2011.igem.org/Team:Arizona_State/Project/L_innocua#Construction successfully ligated] into pRSF Duet along with Cas9 and tracrRNA precursor, ready for inducible CRISPR-Cas expression. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 23:32, 2 October 2011
T7LRSR (Listeria innocua CLIP11262)
This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Confirmation of Part Length
Use of this BioBrick
This part has been [http://2011.igem.org/Team:Arizona_State/Project/L_innocua#Construction successfully ligated] into pRSF Duet along with Cas9 and tracrRNA precursor, ready for inducible CRISPR-Cas expression.