Difference between revisions of "Part:BBa K649200"
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lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. | lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. | ||
| − | The Cre-mediated recombination of this BioBrick had been studied by both ''in vitro'' and ''in vivo'' assay. | + | The Cre-mediated recombination of this BioBrick had been studied by both ''in vitro'' and ''in vivo'' assay, and proved to be work. |
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Revision as of 16:25, 2 October 2011
PlacIQ-lox2272-gfp-lox2272
lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied by both in vitro and in vivo assay, and proved to be work.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 107
Illegal BamHI site found at 13 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 790