Difference between revisions of "Part:BBa K649200:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | PlacIQ + lox2272 + RBS + GFP + T7 + lox2272 | |
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===Source=== | ===Source=== | ||
Latest revision as of 16:08, 2 October 2011
PlacIQ-lox2272-gfp-lox2272
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 107
Illegal BamHI site found at 13 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 790
Design Notes
PlacIQ + lox2272 + RBS + GFP + T7 + lox2272
Source
Both lox2272 are annealed oligonucleotides. The information of lox2272 sequence was gain from the reference below.
References
Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.