Difference between revisions of "Part:BBa K649200:Design"
(→‎References)
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===References===
 
===References===
 +
Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.

Revision as of 16:07, 2 October 2011

PlacIQ-lox2272-gfp-lox2272


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790


Design Notes

test1


Source

Both lox2272 are annealed oligonucleotides. The information of lox2272 sequence was gain from the reference below.

References

Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.