Difference between revisions of "Part:BBa K649104"
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<partinfo>BBa_K649104 short</partinfo>
 
<partinfo>BBa_K649104 short</partinfo>
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We measured the transcriptional activity of our lsrA promoter by introducing a gfp gene downstream of the promoter.  Its fluorescence intensity was much higher than that from a promoter-less GFP negative control plasmid, showing that our new lsrA promoter works.  
 
We measured the transcriptional activity of our lsrA promoter by introducing a gfp gene downstream of the promoter.  Its fluorescence intensity was much higher than that from a promoter-less GFP negative control plasmid, showing that our new lsrA promoter works.  
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We improved previous lsrA promoter(BBa_K117002).[https://parts.igem.org/Part:BBa_K117002:Experience our assay of BBa_K117002]
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For more information, see our work in Tokyo_Tech 2011 wiki
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:26, 2 October 2011

PlsrA-RBS-gfp


We measured the transcriptional activity of our lsrA promoter by introducing a gfp gene downstream of the promoter. Its fluorescence intensity was much higher than that from a promoter-less GFP negative control plasmid, showing that our new lsrA promoter works.


We improved previous lsrA promoter(BBa_K117002).our assay of BBa_K117002


For more information, see our work in Tokyo_Tech 2011 wiki


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770