Difference between revisions of "Part:BBa K676006:Design"
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The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence. | The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence. | ||
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[[Image:Primer_Design_for_Exonuclease.jpg]] | [[Image:Primer_Design_for_Exonuclease.jpg]] | ||
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===Source=== | ===Source=== | ||
| − | T5 | + | T5 bacteriophage genome - 1145 to 2020 bp |
===References=== | ===References=== | ||
| + | |||
| + | 1. Kaliman, A.V., et al., Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5. FEBS Lett, 1986. 195(1-2): p. 61-4. | ||
| + | |||
| + | 2. Wang, J., et al., Complete genome sequence of bacteriophage T5. Virology, 2005. 332(1): p. 45-65. | ||
Latest revision as of 03:23, 2 October 2011
T5 Phage Exonuclease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 600
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence.
Source
T5 bacteriophage genome - 1145 to 2020 bp
References
1. Kaliman, A.V., et al., Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5. FEBS Lett, 1986. 195(1-2): p. 61-4.
2. Wang, J., et al., Complete genome sequence of bacteriophage T5. Virology, 2005. 332(1): p. 45-65.