Difference between revisions of "Part:BBa K676006:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Design primers for the PCR to extract the DNA sequence as well as primers for the site directed mutagenesis to remove the XbaI restriction site at the 241th bp position in the 892bp T5 exonuclease DNA sequence.
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The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence.
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[[Image:Primer_Design_for_Exonuclease.jpg]]
  
 
===Source===
 
===Source===

Revision as of 03:20, 2 October 2011

T5 Phage Exonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The exonuclease is coded for by the D15 gene in the T5 bacteriophage genome. The gene was cloned out by PCR using designed primers. After cloning, site-directed mutagenesis was carried out to remove the XbaI restriction site loacted at the 225th to 230th nucleotide in the DNA sequence.


Primer Design for Exonuclease.jpg

Source

T5 Bacteriophage

References