Difference between revisions of "Part:BBa R0079:Experience"

(→‎User Reviews)
(→‎User Reviews)
Line 54: Line 54:
  
  
−
We improved this part. LasI promoter([https://parts.igem.org/Part:BBa_K649001:Experience BBa_K649000]) which we constructed was successfully regulated by 3O-C12-HSL.
+
We improved this part. LasI promoter([https://parts.igem.org/Part:BBa_K649001:Experience BBa_K649000]) which we constructed was more successfully regulated by 3O-C12-HSL.
  
  
 
|}
 
|}
 
|};
 
|};

Revision as of 23:00, 1 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_R0079

User Reviews

UNIQe557f4a0e8b58831-partinfo-00000000-QINU UNIQe557f4a0e8b58831-partinfo-00000001-QINU

No review score entered. NYMU-Taipei 2009

NYMU 2009-09-29.png We have characterised the strength of the promoters pCI, p22, pLux, pLas relative to pCI. More details are available at the [http://2009.igem.org/Team:NYMU-Taipei/Project/Promoter_Strength_Testing NYMU-Taipei iGEM09 wiki].
;

No review score entered. Northwestern 2011

The Northwestern iGEM team used this part as a unit within our Pseudomonas Aeruginosa biosensor. When this LasR/PAI regulated promoter is induced at varying concentrations of PAI in the presence of excess LasR, we observed GFP fluorescence in accordance with the graph below.


LasR graph.jpg

|};

•••

Tokyo-tech iGEm 2011

Judging from Northwestern iGEM 2011 team's data, in the presence of 3O-C12-HSL, the culture showed about 3-fold higher fluorescence intensity than that in the absence of 3O-C12-HSL.


We improved this part. LasI promoter(BBa_K649000) which we constructed was more successfully regulated by 3O-C12-HSL.


|};