Difference between revisions of "Part:BBa K676004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter). | + | Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter) and pSB1C3 plasmid backbone. |
[[Image:UCL-Content-Experiment_Plan_for_Supercoilometer.jpg]] | [[Image:UCL-Content-Experiment_Plan_for_Supercoilometer.jpg]] | ||
Revision as of 08:45, 1 October 2011
Supercoilometer
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter) and pSB1C3 plasmid backbone.
Source
TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M).
References
1. Straney, R., R. Krah, and R. Menzel, Mutations in the -10 Tataat Sequence of the Gyra Promoter Affect Both Promoter Strength and Sensitivity to DNA Supercoiling. Journal of Bacteriology, 1994. 176(19): p. 5999-6006.
2. Pemberton, I.K., et al., The G+C-rich discriminator region of the tyrT promoter antagonises the formation of stable preinitiation complexes. Journal of Molecular Biology, 2000. 299(4): p. 859-864.
3. Lamond, A.I., Supercoiling response of a bacterial tRNA gene. Embo Journal, 1985. 4(2): p. 501-7.
4. Bowater, R.P., D.R. Chen, and D.M.J. Lilley, Modulation of Tyrt Promoter Activity by Template Supercoiling in-Vivo. Embo Journal, 1994. 13(23): p. 5647-5655.
5. El Hanafi, D. and L. Bossi, Activation and silencing of leu-500 promoter by transcription-induced DNA supercoiling in the Salmonella chromosome. Molecular Microbiology, 2000. 37(3): p. 583-594.
6. Bossi, L., et al., The supercoiling sensitivity of a bacterial tRNA promoter parallels its responsiveness to stringent control. Embo Journal, 1998. 17(8): p. 2359-2367.