Difference between revisions of "Part:BBa K549001:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K549001 short</partinfo>
 
<partinfo>BBa_K549001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Has to be used in E. coli, because Regulator RcnR (E. coli) is needed.  
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This part should be expressed in '' E. coli'', in order to use the ''rcnA/rcnR''-System. The regulator rcnR is naturally expressed in ''E.coli'' and has not to be added to the cells.
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===Source===
 
===Source===
  
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GFP is from E0040, prcnA is from genemic DNA (E. coli K12)
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GFP is from E0040, prcnA is from genomic DNA (''E. coli K12'')
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===References===
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Latest revision as of 18:08, 30 September 2011

Ni(II) dependent rcnA-promoter and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863


Design Notes

This part should be expressed in E. coli, in order to use the rcnA/rcnR-System. The regulator rcnR is naturally expressed in E.coli and has not to be added to the cells.


Source

GFP is from E0040, prcnA is from genomic DNA (E. coli K12)