Difference between revisions of "Part:BBa K549001:Experience"

Revision as of 17:44, 30 September 2011

Our first experiment with this BioBrick showed positive results. Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer. For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.

Transformed and induced E. coli cells, cultivated in LB-media (containing Ni(II)). left-hand side: phase contrast right-hand side: GFP-excitation;

Applications of BBa_K549001

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UNIQa00db2fde7d87f93-partinfo-00000000-QINU UNIQa00db2fde7d87f93-partinfo-00000001-QINU

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+Our first experiment with this BioBrick showed positive results. Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer.
+For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.
-Experience:
+[[Image:mikroskopdurchlicht.jpg|400px|phase contrast microscopy showing with BBa_K549001 transformed E.coli cells]][[Image:mikroskopgfp.jpg|400px|]]
-works
-[[Image:mikroskopdurchlicht.jpg|450px]][[Image:mikroskopgfp.jpg|450px]]
 Transformed and induced ''E. coli'' cells, cultivated in LB-media (containing Ni(II)). left-hand side: phase contrast right-hand side: GFP-excitation;