Difference between revisions of "Part:BBa K646004"

Revision as of 13:26, 30 September 2011

T7 promoter with Hemeoxygenase

Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available.

Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K646004 short</partinfo>
-Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with S and P and ligating it to the HO insert also digested with S and P.
+Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available.
-Successful expression should result in green colonies due to the presence of biliverdin.
+Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1).
 <!-- Add more about the biology of this part here