Difference between revisions of "Part:BBa K646003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K646003 short</partinfo> | <partinfo>BBa_K646003 short</partinfo> | ||
| − | Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with | + | Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. |
Successful expression should result in green colonies due to the presence of biliverdin. | Successful expression should result in green colonies due to the presence of biliverdin. | ||
Revision as of 13:19, 30 September 2011
T7 promoter with Hemeoxygenase
Using parts BBa_K646000 and BBa_I719005, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI.
Successful expression should result in green colonies due to the presence of biliverdin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]