Difference between revisions of "Part:BBa K625002"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K625002 short</partinfo>
 
<partinfo>BBa_K625002 short</partinfo>
  
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This is an adapted version of the Pu promoter BBa_I723020. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this biobrick is cloned without scar), a double stop codon was inserted at the end of the promoter.
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This is an adapted version of the Pu promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_I723020 BBa_I723020]. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this biobrick is cloned without scar), a double stop codon was inserted at the end of the promoter.
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==='''Characterization'''===
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===Experimental setup===
  
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A
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:53, 30 September 2011

Pu promoter long version with stop codon

This is an adapted version of the Pu promoter BBa_I723020. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this biobrick is cloned without scar), a double stop codon was inserted at the end of the promoter.

Characterization

Experimental setup

A

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]