Difference between revisions of "Part:BBa K238013:Experience"
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<I>Glasgow iGEM 2011</I> | <I>Glasgow iGEM 2011</I> | ||
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We coupled the bluf promoter to YFP and measured intensity after induction with white light. We found that expression showed a 2.4* increase in fold induction when compared with darkness as a control. | We coupled the bluf promoter to YFP and measured intensity after induction with white light. We found that expression showed a 2.4* increase in fold induction when compared with darkness as a control. | ||
The promoter demonstrated leaky expression with no light induction. | The promoter demonstrated leaky expression with no light induction. | ||
| − | [[Image: | + | [[Image:Gla_YFP_Results.jpg]] |
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The image above demonstrates expression of YFP ligated to an RBS and the bluf promoter. The images are taken from two samples, one which was grown in darkness and the other grown with light present. The samples were shaken overnight at room temperature and the intensity of expression was measured. (See graph below) | The image above demonstrates expression of YFP ligated to an RBS and the bluf promoter. The images are taken from two samples, one which was grown in darkness and the other grown with light present. The samples were shaken overnight at room temperature and the intensity of expression was measured. (See graph below) | ||
| − | Scale = | + | Scale = 10 microns. |
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| + | 1st Fluorescence filter : 515-565nm (left column) | ||
| − | Fluorescence filter : | + | 2nd Fluorescence filter : 470/20 nm (right column) |
[[Image:Bluf graph.jpg]] | [[Image:Bluf graph.jpg]] | ||
Revision as of 23:52, 29 September 2011
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BBa_K238013 Glasgow iGEM 2011 |
Glasgow iGEM 2011 We coupled the bluf promoter to YFP and measured intensity after induction with white light. We found that expression showed a 2.4* increase in fold induction when compared with darkness as a control. The promoter demonstrated leaky expression with no light induction.
The image above demonstrates expression of YFP ligated to an RBS and the bluf promoter. The images are taken from two samples, one which was grown in darkness and the other grown with light present. The samples were shaken overnight at room temperature and the intensity of expression was measured. (See graph below) Scale = 10 microns. 1st Fluorescence filter : 515-565nm (left column) 2nd Fluorescence filter : 470/20 nm (right column)
The graph clearly shows that expression has been significantly induced by white light for expression with the bluf domain. When averaged out, the total induction shows an increase in YFP fluorescence by a 2.4 fold level, however, the promoter does exhibit some leaky expression. This was the only light-induced promoter, out of the 3 that the Glasgow iGEM 2011 team trialled, which demonstrated any measurable increase in expression.
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