Difference between revisions of "Part:BBa K640002:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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oriT, or origin of transfer, is a DNA platform which allows for the transfer of plasmid DNA from one organism to another. Relaxases and nickases are responsible for cutting the DNA, host cell polymerases facilitate rolling circle replication of one of the plasmid strands in the host, and <i>rep</i> genes mediate transfer to the receptor organism. This oriT part is an alternate form of BBa_J01003, and allows DNA transfer between <i>E. coli</i> and <i>Pseudomonas</i>.
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This parts was amplified out of plasmid DNA and the biobrick prefix and suffix were added via polymerase chain reaction.
  
 
===Source===
 
===Source===
  
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This part was obtained from Dr. Tung.
 
===References===
 
===References===

Latest revision as of 20:10, 29 September 2011

oriT - Interspecies origin of transfer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This parts was amplified out of plasmid DNA and the biobrick prefix and suffix were added via polymerase chain reaction.

Source

This part was obtained from Dr. Tung.

References