Difference between revisions of "Part:BBa K274004:Experience"
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When digested with BamHI and NotI, the insert did not digest as expected, only a 2kb band (backbone) and ~6kb band were visible. | When digested with BamHI and NotI, the insert did not digest as expected, only a 2kb band (backbone) and ~6kb band were visible. | ||
| − | == | + | === Characterisation by 2011 iGEM NCTU_FORMOSA === |
This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts. | This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts. | ||
Revision as of 11:53, 29 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K274004
User Reviews
UNIQ18e0442c9feba6bc-partinfo-00000000-QINU UNIQ18e0442c9feba6bc-partinfo-00000001-QINU
E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.
Karina Arnesen, undergraduate, Alberta iGEM 2010
When digested with BamHI and NotI, the insert did not digest as expected, only a 2kb band (backbone) and ~6kb band were visible.
Characterisation by 2011 iGEM NCTU_FORMOSA
This plasmid did not cut with Xba I restriction site in our hands. As such we could not use it to assemble after other parts.
We mutated the sequence to correct the Xba I restriction site .
And the sequence is made of vioABDE not vioABCE