Difference between revisions of "Part:BBa K672000"

Revision as of 06:35, 29 September 2011

THERMOGENIC RESPONSE NUTRIENT BIOSENSOR

The Bristol BBa_k381001 encodes an expression of GFP in response of Nitrate or Nitrites that expresses fluorescent signals upon nutrient detection, its principal application is that allows farmers to quantify soil nutrient content. The BBa_k410000 is a fusion of the HydB cold shock promoter, the OmpA a signal peptide, and AOX 1 . AOXa is a alternative oxidase found in Sacred Lotus. This is going to produce a increase of heat above ambient temperature as follow:

· From 20°C to 30°C the HydB promoter will express. · Approximately at 37°C the promoter will stop his expression.

By combining BBa_k38100 and BBa_k410000 we expected our part , the BBa_K672000 , to be able to sense nitrate and nitrites even after experiencing a cold shock at 20º C, making our BB employable in other places where the temperatures can be as low as 15º C.

@@ Line 1: / Line 1: @@
 == '''THERMOGENIC RESPONSE NUTRIENT BIOSENSOR''' ==
-The original BioBrick that senses the levels of nitrates in soil developed by Bristol iGEM Team 2010, was designed to operate at room temperatures, especially for 37 ° C using the PyeaR BioBricks promoter.
+The Bristol BBa_k381001 encodes an expression of GFP in response of Nitrate or Nitrites that expresses fluorescent signals upon nutrient detection, its principal application is that allows farmers to quantify soil nutrient content. The BBa_k410000 is a fusion of the HydB cold shock promoter, the OmpA a signal peptide, and AOX 1 . AOXa is a alternative oxidase found in Sacred Lotus. This is going to produce a increase of heat above ambient temperature as follow:
-Our composite seeks to extend the range of optimal functioning of the sensor developed by Bristol at temperatures below 20 ° C, as many of the crops in highlands (coffee and fruits), are in this range. To resolve this problem, we found that the use of the AOX enzyme (Alternative oxidase) gene responsible for thermogenesis in the sacred lotus, mainly used in Synthetic Biology to generate an increase in periplasmic temperature, produced an interesting increase in the growth and downsizing bacterial densities in temperature. This part uses a HybB promoter for "cold shock".
+· From 20°C to 30°C the HydB promoter will express.
-Our new "device" combines the two parts, the AOX periplasmatic heat generator (BBa_K410000) and the Nitrates biosensor (BBa_K381001), to create That We Call The Renbo, to ensure that our meters Nutrient operate at low temperatures.
+· Approximately at 37°C the promoter will stop his expression.
+By combining BBa_k38100 and BBa_k410000 we expected our part , the BBa_K672000 , to be able to sense nitrate and nitrites even after experiencing a cold shock at 20º C, making our BB employable in other places where the temperatures can be as low as 15º C.
 == '''SubParts:''' ==
 [[Image:BBCOMBINATION.jpg]]